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Journal: World Journal of Gastrointestinal Oncology
Article Title: Regulator of chromosome condensation 1 promotes hepatocellular carcinoma proliferation via cell-division-cycle-associated-8 dependent phosphoinositide 3-kinase/protein kinase B signaling
doi: 10.4251/wjgo.v17.i6.106080
Figure Lengend Snippet: Regulator of chromosome condensation 1 regulates phosphoinositide 3-kinase/protein kinase B/cyclin-dependent kinase inhibitor 1a pathway through cell division cycle-associated 8 in hepatocellular carcinoma. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. A: Immunoblotting analysis of phosphatidylinositol - 4,5 - bisphosphate 3 - kinase catalytic subunit alpha (PIK3CA), protein kinase B (Akt), p-Akt and cyclin-dependent kinase inhibitor 1a (p21) in vector and regulator of chromosome condensation 1-overexpressing Huh-7 cells. Actin was used as the loading control; B: Quantification of PIK3CA, Akt, p-Akt and p21 expression normalized to actin; C: Immunoblotting analysis of PIK3CA, Akt, p-Akt and p21 in small interfering RNA negative control and regulator of chromosome condensation 1-knockdown Huh-7 cells. Actin was used as the loading control; D: Quantification of PIK3CA, Akt, p-Akt and p21 expression normalized to actin; E: Immunoblotting analysis of PIK3CA, Akt, p-Akt and p21 in different treatment Huh-7 cells. Actin was used as the loading control; F: The quantification of PIK3CA, Akt, p-Akt and p21 expression normalized to actin. RCC1: Regulator of chromosome condensation 1; siNC: Small interfering RNA negative control; CDCA: Cell division cycle-associated; PIK3CA: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; Akt: Protein kinase B.
Article Snippet: The anti-β-actin (66009-1-Ig, western blot (WB): 1:10000), anti- RCC1 (22142-1-AP, WB: 1:2000), anti-CDCA8 (12465-1-AP, WB: 1:1000), anti-minichromosome maintenance complex component (MCM) 4 (13043-1-AP, WB: 1:1000), anti-CDCA5 (67418-1-AP, WB: 1:3000),
Techniques: Western Blot, Plasmid Preparation, Control, Expressing, Small Interfering RNA, Negative Control, Knockdown